Jun. 4 at 11:31 PM
$CADL There have been a lot of comments today that sound like they're from people that have never met a HEK293. Well, I knew JEK293, and you sirs, are no JEK293.
First thing to remember is that the start of adenoviral production is with a manufactured plasmid. Unless you're a moron that hasn't backed up your hard drive for 10 years (and if you're reading this in France, you know who you are), you should have the DNA sequence of the plasmid recorded in multiple places. So even if your original lab were to be hit by a relativistic impactor and turned to plasma, that sequence should still be in the cloud somewhere and also tattooed onto your back (adenoviruses are only ~36,000 characters long).
And that DNA sequence is what determines what comes out of the HEK293 cells, even if it's a decade later. The CMC isn't going to have any trouble making a functional equivalent of the original garage batch of CAN-2409.
https://blog.addgene.org/adenoviral-vector-production-and-troubleshooting
